![]() ![]() The transcriptome analysis provided a rich genetic resource for gene discovery related to salt tolerance in Maple, and in closely related species. The three most significantly enriched pathways were plant hormone signal transduction, arginine and proline metabolism, and photosynthesis. Moreover, a methyltransferase-related gene was detected among the first 10 most upregulated genes. Two MYB-related proteins and one LEA protein were detected among the first 10 most downregulated genes. Among these DEGs, 125 were upregulated and 178 were downregulated genes. Importantly, 181,769 genes were detected in at least one library, and 303 differentially expressed genes (DEGs) were identified between salt-sensitive and salt-tolerant genotypes. 14,090 simple sequence repeats and over 500,000 single nucleotide polymorphisms were identified, which represent useful resources for marker development. The N50 length of the unigenes was 738 bp, and their total length over 102.66 Mb. Approximately 146.4 million paired-end reads, representing 181,769 unigenes, were obtained. In this study, we performed comprehensive analyses of the salt tolerance in two genotypes of Maple using RNA-seq. However, the limited availability of Maple genomic information has hindered research on the mechanisms underlying this tolerance. ![]() ![]() Salt stress affects the normal growth of the Maple leaf directly, leading to loss of esthetic value. Maple ( Acer palmatum) is an important species for landscape planting worldwide. ![]()
0 Comments
Leave a Reply. |